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Zenit G-Sight

The information to be found in this section is intended for the exclusive use of healthcare professionals.

HEp-2 CELL SUBSTRATE SLIDES

All our substrate slides locally distributed are compatible with the A. Menarini Diagnostics Zenit series platforms and can be assessed either visually with a fluorescence microscope or automatically with the A. Menarini Diagnostics interpretation system Zenit G Sight.
  

CHARACTERISTICS

  • Significant number of cells in mitosis
  • Optimized cell morphology
  • Large nucleus for easy pattern recognition
  • Optimal substrate for the detection of antibodies targeting cytoplasmic antigens such as antigens associated with ribosomal P, tRNA synthetase (Jo-1), mitochondria and smooth muscle
  • ANA positive control provided
  • Substrate provided in accordance with CLSI standard guidelines

ORDERING INFORMATION

Our products are conveniently available in all parts of Europe through a network of local A. Menarini Diagnostics branches. Please contact your nearest branch for additional information and order placing. 

DIAGNOSTIC SIGNIFIANCE OF ANTI-NUCLEAR ANTIBODIES


IIF staining pattern Nature of antigen Associated disease  
 Homogeneous/Peripheral  Deoxyribonucleoprotein
DNA 
 SLE with renal involvement
SLE 
 
 Nucleolar  4S-6S RNA probably U3 RNA  Scleroderma  
 Speckled  RNP
Sm
SS-A/SS-B
Scl-70
 SLE or Mixed Connective Tissue Disease
SLE
SLE or Sjögren’s
Scleroderma
 
 Centromere  Inner and outer plates of kinetochore  CREST Syndrome  
 Cytoplasmic/Mitochondrial  70 kd mitochondrial protein  primary biliary cirrhosis  


Anti-nuclear antibody (ANA) detection: Zenit G Sight vs visual assessment


 ANAs were determined by IIF using HEp-2 cells as a substrate. Tests were conducted blinded on 120 sera from patients with Connective Tissue Diseases or other autoimmune diseases and healthy subjects. Results were evaluated by three independent expert physician examiners and compared with evaluation by the Zenit G Sight automated interpretation system. Excellent agreement (100%) was obtained for the differentiation of positive and negative samples. Discrepancies were observed only in sera identified by Zenit G Sight system as “doubtful negative”: 8% of these were reported “positive speckled” by examiners.