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Zenit AMiDot

The information to be found in this section is intended for the exclusive use of healthcare professionals.

General Description

Pre-designed microdot arrays for a panel of Autoimmune Diseases:

  • Systemic Autoimmune Disease (SLE, SSc, MCTD, Sjögren’s Syndrome, Raynaud’s) - product codes 43895, 43896
  • Autoimmune Vasculitis (arteritis, Wegener Granulomatosis, Churg-Strauss syndrome, Goodpasture’s disease) - product codes 43897, 43898
  • Idiopathic In ammatory Myopathies (polymyositis, dermatomyositis) - product codes 43891, 43892
  • Gastrointestinal Autoimmune Disease (gastritis, ulcerative colitis, coeliac disease, IBD) - product codes 43889, 43890
  • Autoimmune Liver Disease (autoimmune hepatitis, PBC) - product codes 43893, 43894

TheZENIT•AMiDot testis based on the Immunoassay principle. It consists of aReagent ZENIT•AMiDot Reagent Kitcontaining conjugate, sample diluent and wash buffer, and aZENIT•AMiDot Slide Packcontaining glass microscope slides with 4 or 8 wells defined by a hydrophobic mask. These wells are coated with an array of autoimmune antigens, calibrators and controls.
Each ZENIT•AMiDot panel-specific slide can assay 4 or 8 patients, one patient per well


Each well contains:

  • a 9 x 9 array of up to 38 different antibodies or antigens measured in duplicate
  • a calibration curve for each antigen
  • assay controls
ZENIT•AMiDot slides ensure quantitative, reliable and reproducible results

  • An anti-human IgA status control (a negative result may indicate IgA deficiency)
  • An anti-human IgG status procedural control
  • Intra well calibration for IgG and IgA provides patient-specific calibration

Principle

ZENIT•AMiDot slides can be run either manually or on automated IFA slide processors

The principal steps of the method are:

Serum sample dilution (1/80) and incubation with autoimmune antigen array fixed on glass slides for 30 min in a moisture chamber at RT
Incubation with flurophore conjugated goat anti-human IgG/IgA for 30 min at RT
Assessment: automated analysis of slides


Signal intensity is directly proportional to the amount of antibody present in the sample. Results are expressed in U/ml or IU/ml.

Automation

ZENIT•AMiDot slides are completely compatible with:


ZENIT•AMiDot slides may be read as soon as they are prepared. However, no significant loss of intensity occurs if reading is delayed for up to 48 hours. In this case, ZENIT•AMiDot slides should be stored in the dark, at room temperature.